AUTOMATED DNA SEQUENCERS FOR SALE
Three ABI 373 DNA sequencers, all currently in use and under service
contract are available for sale. Please contact Dr. Eric Rappaport
(rappaport at email.chop.edu) with offers or questions. Details of the
equipment configuration follow:
Two 373 units with Stretch upgrades:
Filters - 5-filter wheel (can choose between 2 different sets of 4
different dyes).
Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7
polymerase sequencing chemistry. All current Genescan
chemistries, including microsatellite panels, primers labeled
during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or
(6-Fam, Hex, Tamra, and Rox).
Gel lengths - Multiple gel (well-to-read) lengths including 48 cm
(standard long-read [>650 nt] sequencing length), 34 (shorter sequence
reads [550-600 nt] or high-resolution Genescan applications), 24
(microsatellite applications) 12 (lower-resolution Genescan
applications) or 6 (lower-resolution Genescan applications).
Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can
be used for faster analysis of shorter sequences).
One 373 with multiple gel length (Leon) configuration:
Filters - 5-filter wheel (can choose between 2 different sets of 4
different dyes).
Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7
polymerase sequencing chemistry. All current Genescan
chemistries, including microsatellite panels, primers labeled
during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or
(6-Fam, Hex, Tamra, and Rox).
Gel lengths - Multiple gel (well-to-read) lengths including 24 cm (shorter
sequence reads [400-500 nt] or microsatellite applications) 12
(lower-resolution Genescan applications) or 6 (lower-resolution
Genescan applications).
Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can
be used for faster analysis of shorter sequences).
Computers and Software
Unit 1 (Stretch) - Mac IIcx with 8 mB RAM/230 mB hard drive
Unit 2 (Stretch) - Mac IIci with 8 mB RAM/100 mB hard drive
Unit 3 (Leon) - Mac IIci with 8 mB RAM/120 mB hard drive
All units are equipped with latest versions of analysis and collection
sofware for running sequencing and Genescan applications on the above
computers. Upgrades for PowerMac are not included.
Service Contracts
All units have been under service contract since the time of their
purchase from ABI and are currently covered under those agreements.
Purchaser would need to have an installation service call from ABI before
initiation of a new service contract could occur.
