This works (honest!)
Cut out band, place in Eppendorf tube with 40 ul of water, leave in fridge
overnight. The resulting eluent makes perfect sequencing template...
In article <8khatf$gss$1 at mercury.hgmp.mrc.ac.uk>,
"Pezzolesi, Marcus" <Marcus.Pezzolesi at joslin.harvard.edu> writes:
> We have recently been having problems with our cycle sequencing and feel
> that the problem lies in the preparation of DNA template for sequencing. In
> the past, we were having quite a bit of success with cutting bands from
> agarose and then using Qiagens Qiaquick spin columns, but this is no longer
> working well. We have also tried PEG precipitation, but also with mixed
> results.
>> Can anyone offer a protocol which has proven to work well?
>> Thanks.
>>> ---
>>
--
Marc Botcherby
Mouse Sequencing Group leader UK-HGMP
m.botcherby at hgmp.mrc.ac.uk
Tel:01223 494555 Fax: 01223 494512
