Duncan Clark wrote:
> Historians believe that in newspost
> <mailman.683.1127317265.29381.autoseq at net.bio.net> on Wed, 21 Sep 2005,
> Phillip San Miguel <pmiguel at purdue.edu> penned the following literary
> masterpiece:
>>> If, however, you are using the strains that Invitrogen normally
>> includes with it's cloning kits--those give us enough trouble that we
>> toss them and buy DH10B electrocompetant cells.
>>> Which strains don't work for you?
>> Duncan
I stick with DH10B, DH5alpha and the XL's where I can. Many other
strains caused this issue (although a long time ago.) Top10 has given me
the broad peak issue.
But to tell the truth, this might have only happen with a miniprep
method we no longer use...
Phillip
