A request was made for a program which will design PCR primers.
I have included the README file from the PRIMER program available via anonymous
ftp from genome.wi.edu. Instructions for obtaining the program on disks are
included in the appended information. PRIMER comes with a pretty extensive man
ual and is pretty user friendly. I'll leave the rest to the following file.
danj at jhuhyg.sph.jhu.edu
PRIMER 0.5: Questions and Answers
What exactly is PRIMER?
PRIMER is a computer program for automatically selecting PCR primers. PRIMER te
PRIMER was written by Steve Lincoln, Mark Daly, and Eric Lander at the MIT Cente
What types of computers does PRIMER run on?
In short: just about anything which supports a standard C language compiler, inc
(1) IBM PC and compatibles. A PC/AT (286) class machine or better with 640K of
(2) Apple Macintosh computers. A Mac II series or SE/30 with a math coprocessor
(3) Most Unix workstations including Sun SPARCStation and DEC DECStation systems
(4) DEC VAX/VMS computers under VMS Version 5 with VAX C.
How do I get PRIMER?
PRIMER is available from a number of sources:
(A) You can get PRIMER from another user, provided you strictly follow the terms
(B) You can download the most recent version off the Internet via anonymous FTP
(C) You can send a request to:
"PRIMER c/o The Lander Lab,
9 Cambridge Center, Cambridge, MA 02142.
Email to "primer at genome.wi.edu" (Internet), or FAX to 617-258-6505
Request a copy of PRIMER, specifying a 3 1/2" floppy disk for either SPARCStatio
Do I really need to compile PRIMER myself, and if so, how?
If you receive a ready-to-run version from us or another user, then you do not.
The C source code we distribute may be compiled using:
(1) Microsoft C 4.0 on IBM PC compatibles. The file MAKEPC.BAT included execute
(2) Apple Macintosh computers with ThinkC 4.0.1. A ThinkC project file has been
(3) DEC VAX/VMS computers under VAX C with VMS 5.x. The file MAKEVAX.COM includ
may have to change the linking procedure depending on your system's configuratio
(4) Unix workstations including Sun SPARCStation and DEC DECStation Systems with
Before compiling the program, you may need to modify configuration information i
What's the fine print?
PRIMER and its documentation are copyright 1991 by the Whitehead Institute for B
PRIMER is available for free use for non-commercial purposes by basic research l
PRIMER may be freely redistributed to other scientists, provided that all of the
Primer is designed to select primers for PCR applications, although it may may a
PRIMER is supplied without any warranty or guaranteed support whatsoever. The W
WHAT ARE ALL THESE FILES WHICH CAME WITH PRIMER?
primer.c PRIMER C Source Code
text.c PRIMER C Source Code
primer.h PRIMER C Source Code
sample.seq Sample Input Sequence File
sample2.seq Longer Sample Input Sequence File
standard.cri Sample PCR Primer Criteria File
sample.rep Sample Repeat Sequence File (Human Alu only)
mouse.rep Sample Repeat Sequence File (Mouse Sequences)
rat.rep Sample Repeat Sequence File (Rat Sequences)
human.rep Sample Repeat Sequence File (Human Sequences)
manual.txt PRIMER Manual text file (no line breaks)
manual.asc PRIMER Manual text file (with line breaks)
readme.txt This Document (text file, no line breaks)
readme.asc This Document (text file, with line breaks)
makepc.bat DOS Batch file to compile PRIMER
makevax.com VAX/VMS Command file to compile PRIMER
makefile Unix Makefile to compile PRIMER
Other files which may be included with specific versions include:
primer PRIMER Executable for Sun SPARCStations
primer.exe PRIMER Executable for PC Compatibles
PRIMER PRIMER Executable for Macintosh
PRIMER Manual PRIMER Manual (Mac Microsoft Word 4.0 Format)
The PRIMER Manual PRIMER Manual (Macintosh Executable)
PRIMER README This Document (Mac Microsoft Word 4.0 Format)
The PRIMER README This Document (Macintosh Executable)
Remember, to legally redistribute PRIMER to anyone else, you must give them unmo
Steve Lincoln and Mark Daly
MIT Center for Genome Research
Whitehead Institute for Biomedical Research
9 Cambridge Center, Cambridge, MA 02142
primer at genome.wi.edu, FAX: 617-258-6505