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Oligonucleotide Tm prediction

vldnaseq at hkucc.hku.hk vldnaseq at hkucc.hku.hk
Thu Feb 4 23:28:33 EST 1993


Dear netters,

I am optimizing the hybridization and washing conditions for single base
mismatch oligonucleotide hybridization experiments.  This is used as a means
to detect mutations.  Is there any software that can predict accurately the
Tm of a complete match / single mismatch under different ionic strengths ?

Wai Ming




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