In article <1993Feb5.122833.1 at hkucc.hku.hk> vldnaseq at hkucc.hku.hk writes:
>>I am optimizing the hybridization and washing conditions for single base
>mismatch oligonucleotide hybridization experiments. This is used as a means
>to detect mutations. Is there any software that can predict accurately the
>Tm of a complete match / single mismatch under different ionic strengths ?
A quick Gopher seacrh tells me that there is a program for the PC
called Melt (by John Nash) which may be what you're looking for.
You can obtain melt.uue by gopher or by anonymous ftp at
Best of luck,
danj at welchgate.welch.jhu.edu