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Tm prediction : more

Bob Gross Robert.H.Gross at dartmouth.edu
Mon Feb 8 17:50:32 EST 1993


In article <1993Feb8.190445.1 at hkucc.hku.hk>
vldnaseq at hkucc.hku.hk writes:

> Naturally, Tm is DNA concentration dependent

Shouldn't Tm be DNA concentration INdependent? It just represents the
energy needed to pull the two strands of DNA apart - that is
independent of how many molecules of DNA there are. Reassociation of
the two strands obviously is concentration dependent.


Bob Gross
Dept. of Biology, Dartmouth College
bob.gross at dartmouth.edu




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