In article <1993Feb21.233647.8330 at news.cs.brandeis.edu>, ahouse at hydra.rose.
brandeis.edu (Jeremy John Ahouse) writes:
> I too think that using a hybrid approach seems to make the most sense
> right now. Especially for those with internet access. On the mac:
> (1) assembling with sequencher is straightforward
> (and sequencher can read and write in many formats),
> (2) analyze with intron/exon guessers like GRAIL (by email server)
> (3) search with BLAST (by email server)
> etc. etc.
YES! I think lot's of people do this, but you do need a reasonable amount of
cross-programme expertise of course. I am interested in the interactions
between viral and cellular proteins - a process often involving coevolution
and mimicry - and off the top of my head I guess we have worked like this;
1. Browse GenBank wrt your gene of interest with Blast
2. Assemble potentially interesting alignments with GCG pileup etc.
(definitely preferred round here to ClustalV)
[Export the business parts of alignments to MacDraw etc for publication
quality graphics via VersatermPro]
3. Design and construct (co)expression vectors in GCK
4. Start the *real* work of understanding what is really going on....
Will these systems ever have a common, easy, affordable GUI?
Institute of Virology