> In article <1992Dec30.185104.9506 at alw.nih.gov> usdin at helix.nih.gov (Ted Usdin) writes:
> >Weiner and Scheraga (CABIOS, vol 5 pp191-198, 1989) described a Macintosh
> >program (Gene.Design.?) to assist in introducing restriction sites into DNA without changing the amino
> >acid sequence. Does anyone know where I can get a copy of this, or a similar
> >program that runs on a Macintosh?
>> There is a similar program which runs on a PC called OligoMutantMaker.
> It is available by anonymous ftp from:
> Host fly.bio.indiana.edu
>> Location: /molbio/ibmpc
> FILE -rw-r--r-- 8540 May 14 1991 oligo.doc
> FILE -rw-r--r-- 231882 May 14 1991 oligo.uue
> Dan Jacobson
>danj at welchgate.welch.jhu.edu
I would like to add that we have also developed a PC compatible
program called SILMUT for identifying regions for the introduction of
restriction enzyme sites by silent mutagenesis. Although we have used
the same principle as detailed in the above two programs our approach
was highly simplified. This programs just tells you what restriction
enzyme/s can be introduced by silent mutagenesis in a specific sequence
of amino or nucleic acid. This program is most useful for those who are
contemplating introduction of RE sites to generate casette vectors or
for swapping specific fragments of a gene in a construct.
The program is also available on fly.bio.indiana.edu in the
directory molbio/ibmpc as two files: Silmut.xxx.
The work has been published in Biotechinques 12: 882-884 (1992).
The program sets include source codes for the program which has been internally
documented. The programs work in PC and unix machines but if anyone can
make it work on a Mac, we would welcome that.
bsh at med.pitt.edu