PROSA - PROtein Structure Analysis
Version 1.0 for SGI Iris/Indigo
Copyright (C) by CAME - Center of Applied Molecular Engineering
PROSA analyses the energy distribution in proteins. If you are a protein
X-ray crystallographer, an NMR-spectroscopist or (beware) a protein
structure predictor this program may be of interest to you.
Suppose that you are confronted with a NATIVE protein structure (i.e. a
protein in its natural functioning state). The structure may have been
determined by X-ray, NMR, molecular modelling or it may be a structure
which was designed to fool specialists in protein structure theory.
How could you tell whether the structure is correct or incorrect? Note
that even experimentalists are human beings and prone to errors. All of
them are honest and trustworthy people. But if you base your experiments
on published structures you are better off if you check the structures
as far as possible before you start wasting your time. If you are going to
use the results of protein modelling or prediction then you are in a bad
PROSA is a programm which analyses the energy distribution in protein
structures as a function of sequence position. Native structures have
balanced energy distributions - faulty structures have high energy
peaks or are strained all over the place. So much for the theory. In
case you are really interested please consult the references at the end
of the manual. Just look at a few native graphs and you get the point.
In interpreting the results produced by PROSA keep in mind the following:
BE SUSPICIOUS OF EXPERIMENTAL DATA
BUT NEVER TRUST THEORETICAL METHODS
PROSA runs on Silicon Graphics Workstations with IRIX 4.0.1 or higher.
You can get the latest version of the program by anonymous ftp from
Gundi.came.sbg.ac.at (220.127.116.11) , PROSA/
Please get and read the README file.