In article <k-tougu-020694134859 at e-mac-040.ski.mskcc.org>, k-tougu at mskcc.org (Killu Tougu) writes:
> Hi Netters-
>> We are currently demo'ing a copy of a commercial primer optimization
> program by NBI called "Oligo 4.0" that finds optimal primer sites for PCR,
> sequencing, hybridizations etc. While this program seems to work pretty
> nicely, we're still interested in what else is out there (and if any such
> programs would actually be freebies, since cost is an issue). Also, we'd
> greatly appreciate any comments on the utility and/or weaknesses of this or
> other such programs. We'd appreciate your answers at the address
>ktougu at mskcc.org or in this list!
>> Thanks in advance,
> Killu Tougu,
> mol. biol. grad student,
> Sloan-Kettering Institute
> New York
If the (rather exhorbitant) price tag of Oligo 4.1 is rather off-putting
but you like the style then you can collect a copy of Oligo 2.0 which is
the last non-commercial version.
ftp to nemo.life.uiuc.edu and grab /lim/uploads/oligov2.zip
(thanks to Ducan Clark of GeneSys for providing this)
Another freebie worth a look is Primer 0.5
(Both these are PC software)
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)