I'm trying to locate software that can be used to quantify
hybridization to arrayed colonies that have been spotted, in an interleaved
fashion, on membranes. The colonies will have been spotted on the filter
from 96 well (or denser) microtitre plates in a 3x3 to 5x5 interleaved
pattern (that is, 9 to 25 plates of clones will be spotted on one filter).
The software should be able to read a rasterized image of the autorad,
automatically locate the colonies and write out a file with the colony
positions and the amount of hybridization. If anyone knows of people or
groups using or developing such software, I would appreciate hearing about
them. (For hardware, we prefer something that will run on a Mac or Sun, but
we can handle just about anything.)
Thanks in advance for the help. I'll summarize the responses
back to the newsgroup.
sclark at amgen.com