Does anyone have any recommendations for a good mac based PCR primer design
program? I'd like to find a program that will:
-check for other possible "mispriming" sites in a template with primers
-let me specify one primer, then come up with another one to go with it at
some specified distance range
-predict extinction coefficients (i.e., e260's)
and just otherwise do more than what's included in MacVector.
I'll post a summary of replies.