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Primer hybridization

Peter Kulmburg kulmburg at sun1.ukl.uni-freiburg.de
Thu Jun 30 03:43:30 EST 1994


Dear netters,

I am looking for a (free) program running on a Mac that allows me to
calculate primer hybridization conditions at various temperatures and at
different salt concentrations.

Everybody knows about the formula Tm = 4x(G+C) + 2x(A+T), but it is not
very helpful in using at conditions far from the standard ones.

If the program could estimate the difference in Tm of primers with one or
several mismatches, it would be even better.

Thank you all           Peter

Peter KULMBURG (193.196.226.110    MacName: ktl110.ukl.uni-freiburg.de)
home: Tel./Fax:+49 761 808439   work: Tel.:+49 761 270/7195   Fax:/7177






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