A program in BASIC that can be used to calculate MPNs and
confidence intervals is found in:
Hurley and Roscoe (1983) J. Appl. Bacteriol. 55:159
We use it routinely with good success. It is particularly attractive
because one is not constrained by a fixed number of dilutions, a
fixed number of replicates at each dilution, or dilution by a
fixed amount. It also provides confidence intervals about the estimates.
Marien Science Inst.
Univ. Texas, Austin