I am seeking contact with anyone who has experience with the Radlig
program for analysis of receptor binding kinetics. I have recently
purchased v4.0 (PC version) from Biosoft in the UK. Use of the program
for analysis of my "hot saturation" experiments works well and yields
binding constants compatible with earlier analysis of the data done
without Radlig. However attempts to analyse data from "cold saturation"
experiments do not work at all despite the fact that it is the same
ligand-receptor system and manual analysis of the data yields results in
keeping with the "hot saturation" experiments.
Advice would be greatly appreciated.