If somebody , as I do, uses Oligo 4.0-s to design his primers,
could he be so kind as to share its opinions about this question?:
Is just my impression or the program is extraordinarily, maybe
excessively severe? If I do a combined search, 9 time out of ten
the program does not find two primers which could give a reasonable
probability of success. I use the program default parameters.
Thanks in advance for your answers.