Dear NIH Image users:
I am trying to use this program to analyze levels of protein from a
scanned image of an autoradiogram. After opening the image in NIH
image, I use the rectangular selection tool to select the left most band
(the first lane). Then the mark first lane command is issued. A copy
of the image appears but only of the first lane (of a total of 6 lanes)
with the desired band selected.
1. Is this what should occur? The manual shows a copy of the full
image with the ROI in lane 1 selected.
2. If not, is this a memory problem?
Thanks in advance for any much appreciated advice,