I am trying to collect advices for manual design of primers. Is there any
good netsites out there with this info or could you help me post some
I have an old one regarding purin content - it should not be more than
50% - but is this really true nowadays with pure synthesis chemicals.
Another one is you should have Gs and Cs in the 3'end but not more than 3
Gs in a row.
Than thera are the obvious ones regarding secondary structure and
Maybe you have some more advice to donate??