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curve fitting to estimate RFLP fragment sizes

vargaa at unbc.ca vargaa at unbc.ca
Fri May 22 16:49:54 EST 1998


The software I use for RFLP-analysis, RFLPScan, by Scanalytics, uses four
functions to calculate fragment sizes:	eg:  log linear piecewise.  I
compared all four methods and of course got 4 different sizes for the same
band.

If comparisons between laboratory RFLP databases are necessary, especially
banding topologies, how can datasets be compared if different formulae were
used to estimate fragment sizes?  How can 'fingerprints' be compared if
different methodologies (curve-fittings) were used to estimate band sizes?



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