There have been many replies in this thread to the question of what program
to use for kinetic parameter fitting. Suggestions were (a) use software from
books, (b) KINSIM, (c) Hyper, (d) DynaFit. There are many such programs
around (I'd include Duggleby's DNRP53 as well) but I would really only
reccomend Petr Kuzmic's Dynafit. I'd also strongly reccomend reading his
paper in Anal.Biochem. (v.235, p 260, 1996) as it explains quite
convincingly the problems inherent in kinetic parameter fitting. I'd also
reccomend the following paper: Johnson ML (1992) "Why, when, and how
biochemists should use least squares" Anal.Biochem. 206, 215-225
In short, go with DynaFit which is the best program around for this purpose.
Pedro Mendes Inst. Biological Sciences
prm at aber.ac.uk (home: pedro at enzyme.demon.co.uk) Univ. Wales, Aberystwyth,
http://gepasi.dbs.aber.ac.uk/pedro/prmhome.htm Ceredigion SY23 3DA, U.K.
Author of GEPASI, a biochemical kinetics simulator