Have you tried Scion Image?
It is available from Genamics SoftwareSeek, http://genamics.com/software/
in the Image Analysis section. There may be other programs of use to you
Hope this helps.
Nicole Klapka <klapka at uni-duesseldorf.de> wrote in message
news:7ncn5c$9le at poseidon.rz.uni-duesseldorf.de...
>> I'm looking for software like NIH image for PC . It has to substract
> images and calculate the luminance of each pixel so that the image can be
> converted into a diagram.
>> If You happen to know something like that, please write me.
>> Thank You,