I have used a bioperl module called sigcleave.pm, and compared it
with the signal P web server results, see:
If there are discrepancies I recommend the signalP2 results over
sigcleave. This is all PC based stuff, bioperl requires perl, in
linux this is easy, for windows google search for activeperl (free
from activestate but I can't remeber the url).
Hope this is useful.
"Mr. A. Brown" wrote:
>> I have a small database of mammalian signal peptide sequences. I am
> looking for a method (program) that would allow me to scan these signal
> peptides, in conjuction with a protein I wish to express, so that I can
> select the 'best' signal peptide for that protein. The proteins are to
> be expressed in both COS and CHO cells (hopefully secreted).
>> We run both Macs and PC's in our lab, so programs for either (or both)
> would be welcome, as would web-based systems (Netscape or Explorer).
>> Many Thanks in Advance.
>> Alex Brown,
> e-mail: abrown at nimr.mrc.ac.uk> e-mail: abrown at hgmp.mrc.ac.uk
Nicholas Dickens BSc ARCS
MRC FGU, Department of Human Anatomy & Genetics,
South Parks Road, Oxford, OX1 3QX
Tel: 01865 2(82650)
http://www.ndickens.fsnet.co.uk/nicholas.dickens at human-anatomy.ox.ac.uk