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[TE] EasyPCR and EasyPROBE: Two hypermedia presentations for teaching the principles and and practices of PCR and DNA probes.

ura1335 at citi2.fr ura1335 at citi2.fr
Thu May 16 18:05:36 EST 1996

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EasyPCR and EasyPROBE uses integrated text, lessons, colorful graphics 
and animated demonstrations to illustrate the basic concepts and 
techniques of the Polymerase Chain Reaction (PCR) and DNA probes in a 
highly interactive and entertaining format which makes those aspects 
of Molecular Genetics learning easy and interesting. The programs were 
prepared for science and medical students in their second year at 
university taking their first course in Biochemistry and Genetics. The 
first program, EasyPCR () gives emphasis on the PCR; special topics 
include the basic methods such as the PCR reaction itself, cloning of 
PCR products (termini repair reaction, ligation and transformation), 
screening recombinant colonies by PCR and cycle-sequencing. The main 
subject area of the second HyperCard program, namely EasyPROBE, is the 
use of DNA probes in Molecular Genetics; in this software package, 
special topics include the basic methods such as the restriction 
endonucleases, electrophoresis, labelling and DNA probes, colony and 
plaque hybridization, Southern and Northern blotting, Fluorescent in 
situ Hybridization (FISH), cDNAs and DNA libraries. Both programs have 
been written for the Apple Macintosh (System 6.0.7 and Finder 6.1.7 or 
a newer version) with a color monitor using HyperCard 2.1 or greater 
version and require at least 4 megabytes RAM; PC-Windows9s versions of 
bots packages are also available.

Address for correspondence: 
Bernard  Aral, MD, Ph.D.
Laboratoire de Biochimie Genetique 
CNRS URA1335 Faculte de Medecine Necker
 156, Rue de Vaugirard 75730 Paris Cedex 15
Tel. number: (331) 40615692
Fax number: (331) 40615690
E-Mail: URA1335 at citi2.fr

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