For those of you who have been requesting a Web-available resource for
Restriction Enzyme analysis and mapping, here are the URLs for the WWW
interface to 'tacg', my freely available, command-line restriction enzyme
analysis tool for unixy systems:
The page describing the program in detail:
http://hornet.bio.uci.edu/~hjm/projects/tacg/tacg.main.html
The form for submitting sequence and specifying output:
http://hornet.bio.uci.edu/~hjm/projects/tacg/tacg.form.html
The HTML-ized man page:
http://hornet.bio.uci.edu/~hjm/projects/tacg/tacg.man.html
A preview of the Restriction Enzyme selection form that should be rolled
into the code Real Soon Now:
http://hornet.bio.uci.edu/~hjm/projects/tacg/rebase.select.html
The FTP site for the source code, and executables for a variety of
architectures:
ftp://mamba.bio.uci.edu/pub/tacg/
tacg provides many (most?) of the features of the GCG 'MAP' program and a
few more (including a much better linear format, IMHO).
Enzyme selection via:
Overhang generated (5', 3' or blunt)
Magnitude of recognition sequence (4-cutters + up, 5-cutters + up, etc)
Minimum and Maximum number of cuts
Output in a variety of formats:
Linear map with *exact* cut site indicated
Treat as linear or circular
Translation with 1/3/6 frames in 1 or 3 letter codes
Output width selectable from 60 to 230 characters
GCG-type Ladder Map with 5' (\), 3'(/), and blunt (|) cutters indicated
Pseudo Gel Map with 10 or 100 bp cutoff
Table of Cut Sites
Table of Fragments (Sorted, Unsorted, or both)
Summary Table of Cuts
Strider-like linear summary of infrequent cutters
The WWW interface provides access to all the functions (except the
subsequence selection) of the original program and a few extras:
- an HTML/Forms-based pseudo GUI
- it runs on my system, not yours
- because of the above, it obviously works with Macs and PCs, as well as
anything else that runs a web browser.
- the hyperlinked help and online man page.
- hyperlinked Table of Contents of the results built on the fly
- 3 selectable font sizes (but viewing depends on your browser)
- a few different header sizes for distinctive results separators
Bugs and Oddities:
Altho I didn't research it exhaustively when I started this, my impression
was that Netscape was the only browser that supported file uploads, so this
was writ and debugged with Netscape(2+3). I have no great Browser
preference (besides a philosophical opposition to things small and squishy,
but Netscape allowed me to do what I wanted to do. If Mosaic or IE now allow
file uploads, so much the better.
Altho tacg supports sequence input into the millions of bases, the Web
version supports an upload size of only 100,000 characters. I figure if
you routinely want to do analyses of >100Kb, you can download the program
and run them on your own machine.
While the form allows upload of anything, unless the file is ascii text,
you may get odd or no results. Binary file uploads are not supported, so
don't expect intelligible results from from your MacVector or Lasergene
files.
While the analysis times for 100,000 bases are relatively trivial, the
upload and especially download times can be significant (up to 12x input
size). If it's used enough and the output sizes are significant, I may add
on-the-fly compression (output is especially well-suited to lzw
compression).
Special Thanks go to Fred Criscuolo for the 1st iteration of the Web form
and Tom Boutell for his V1.1 cgic library.
Hope it's of some use,
Cheers
Harry
--
Harry J Mangalam, MolBio+Biochem / Dev+Cell Bio, Rm 4201, BioSciII UC Irvine, Irvine, CA, 92717, (714) 824-4824, fax (714) 824 8598
