With the recent offer to Human Genome Centers of a half-price
discount on large quantity purchases of AmpliTaq, it may be
time to open up a brief discussion about the enzymes of choice
for mapping and sequencing ... especially since other polymerase
manufacturers are matching (or beating) the AmpliTaq pricing.
So : to at least start on the topic of sequencing :
How many folks out there have tried other enzymes for cycle
sequencing using ABI 373's ? Which ones didn't work as well,
which ones worked as well or better, and which one(s) are
you actually using for "production" sequencing purposes ?
[It may help to specify template type, and prep methods .... ]
All responses appreciated !
Greg Lennon
Human Genome Center, L-452
Lawrence Livermore National Lab
Livermore, CA 94550 USA
Email: greg at mendel.llnl.gov
Phone: (510) 422-5711 Fax: (510) 423-3608
