Dear netters,
Does anyone have suggestions on how to get decent titers/DNA
yields with lambda Charon 40 clones in the recA- host K802recA-? I
have tried liquid lysates with multiplicities of infection from 1:100
to 1:500 and 4 - 12 hour incubations, and media including LB/1mM MgCl2
and LB/2 mM CaCl2/10 mM MgSO4/0.1% glucose.
Also, is PEG-8000/NaCl precipitation of lambda inherently
inefficient at low titers? I follow the usual Maniatis protocol.
Thanks!
Nancy Phillips phillinj at sluvca.slu.edu
Pathology phone:(314)577-8782
St. Louis University Hospital fax:(314)268-5120
3635 Vista Ave.
St. Louis MO 63110 USA
