In <45ls5s$18v at clus1.ulcc.ac.uk> "g.coulton" <g.coulton at cxwms.ac.uk>
writes:
>>Can you help me by sending me a good (preferably simple and reliable)
>method for preparing cells for metaphase spreads, prior to chromosome
>paint in situ hybridisation?
>>>Many thanks if you can!!!!!
>>It would be much simpler to just look it up in a cytogenetics book. Do
it the same way for in situ preparation as you would for banding, etc.
Usually prepare slides in the morning, and leave them in a 37 degree
incubator until ready to use them in the afternoon. HK
