>>>------------------------------------------------------------
>>You can do mouse cells the same way as human. Just experiment around
>>abit with colcemid and hypotonic treatment times. If you are using
>>human probes with the mouse chromosomes, you may want to reduce the
>>stringency a bit, but I find that this also increases background, and
>>have usually had good results using the same stringency conditions as
>>for human probes on human chromosomes.
>>>Are you using fibroblast cultures or lymphocytes?
>>>Helene
------------------------------------------------------------------->
>>On a similar note can you do "spreads" with S. cerevisiae chromosomes
?
>
>* David D Morgan
>* University of Aberdeen
>* Aberdeen, SCOTLAND
>* Tel: 01224 273105
*>* Fax: 01224 273144
>* Email: gen159 at abdn.ac.uk
That's a real tough one. From the minimal literature, it appears to be
possible with meiotic chromosomes, rather than mitotic ones. The
trick, then, is to get the little buggers to sporulate. I tried a few
protocols, but either didn't get it to work, or it did, but I just
didn't recognize it under the scope. Don't have the sources handy...I'm
at home, and they're in the lab. I have since moved on to other
projects. I'll take a look tomorrow, and see if I can put my hands on
the sources.
Helene
