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Qiawells for highthrouput template preps

rifat_h rifat_h at icr.ac.uk
Mon Oct 23 08:04:29 EST 1995


Hi,
	I would appreciate any comment regarding what ppl use for their template preps
for dsDNA from high copy plasmids.

I have been trying out various kits and the best one seem to be Qiawells. Promega Wizards
are the worst. However the new Wizard 373 DNA purification system gave 57%.

Having said that the various users have experienced different success rates with Qiawells
however on the whole it did give 85% success.

Any comments regarding the best and most consistant method for double stranded DNA preps
for high throughput sequencing is appreciated.

Thanks.

Rifat 

rifat_h at icr.ac.uk



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