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Craig Venter's Nature supplement paper

rifat_h rifat_h at icr.ac.uk
Sat Oct 28 15:07:23 EST 1995


Hi,

	Has anyone here has any comments on Craig Venter's paper in this October 1995
Nature Supplement entitled "Initial asseesment of human gene diversity and expression
patterns based upon 83 million nucleotides of cDNA sequence".

He goes through the various template preps and the highest success rate is 67.8% for
AGTC the lowest is Promega with 56.9% (Table 3 in the paper). Do you think that 67.8%
is an acceptable success rate for high throughput DNA sequencing. Also he seems to 
measure the percentage success by taking into consideration sequence reactions giving
at least 100bp with fewer than 3% ambiguities. 

I would consider a sequence a successful one if it has 1% ambiguity over 200bp. It
would be appreciated if anyone has any comments regarding how to characterise a 
successful sequence from a failed one.

Cheers.

Rifat Hamoudi,
Institute of Cancer Research,
Sutton.

rifat_h at icr.ac.uk




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