Hello all,
I just bought a new stock of DAPI and I have no idea what would be a
good concentration for FISH (i.e. counterstaining chromosomes).
Does anyone one have a good rule of thumb... ie. for time and conc.
etc.?
As well, what is a good solvent to disolve it in? I think the old stock
we had was in PBS... but I am not sure... I have heard others disolve
the DAPI in whatever anti-fade reagent they are using.
Any and all suggestions are welcome.
Graham Dellaire
McGill University
Experimental Medicine
dellaire at odyssee.net
or popa0206 at po-box.mcgill.ca
