We are using a 96-well protocol which we modified slightly from one posted
by Bruce Roe. You can find his protocol at
http://dna1.chem.uoknor.edu/dsis_seq.html (Bruce, I hope you don't mind my
broadcasting this). We have had very good success using this technique and
generally get a reasonable amount of sequencable DNA.
Peter J. Myler
Seattle Biomedical Research Institute
4 Nickerson Street
Seattle, WA 98109-1651
phone: (206) 284-8846x332
FAX: (206) 284-0313
e-mail: MYLERPJ at U.WASHINGTON.EDU
Matt Ashby <matt at AURORA.XO.COM> wrote in article
<321D2E2F.E67 at mendel.berkeley.edu>...
> Thank you for reading this.
>> What we are looking for is a protocol for doing 96 minipreps of
> transformed E.coli at one time. We would like to start with at least
> 1.5 ml of ON culture and end up with about 50ug plasmid DNA. We are
> using a 2micron plasmid and the E.coli strain DH10B. The DNA from the
> minipreps will be used for sequencing and tranforming of E.coli and
>> Thank you,
>> Doug Gilliland
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