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Ange-Marie Hancock angemari at gibbs.oit.unc.edu
Mon Jun 10 09:16:38 EST 1996

Dear Human Genome Researchers,

Our laboratory has developed strategies to bridge contig gaps occurring 
at human genomic regions which cannot be cloned and/or maintained 
faithfully in bacteria using the large cloning systems (PI/BAC/PAC, 
etc.).  Such strategies derive from the original HAEC technology which 
allows direct cloning of DNA in human cells as Human Artificial Episomal 
Chromosomes (Nature Genetics 8:33-41, 1994; Methods in Molecular Genetics 
8:167-188, 1996).

We have now entered the phase of testing such technology for 
proof-of-concept.  We will provide the human genome community with a 
resource to isolate and sequence refractory contig gaps.  Hence, we are 
interested in hearing from the human genome community about persistent 
and refractory genomic gaps in order to identify potential candidates for 
the HAEC bridging technology.  Suitable candidates are gaps which have 
been thoroughly tested on highly redundant large bacterial and yeast 
libraries and also shown to be missing in libraries prepared with 
different restriction enzymes.

Interested human genome laboratories should send their e-mail reply to 
the attention of Jean-Michel Vos at the following address:  
angemari at gibbs.oit.unc.edu.

Dr. Jean-Michel H. Vos
349 Lineberger Comprehensive Cancer Center
School of Medicine CB#7295
University of North Carolina at Chapel Hill
Chapel Hill, NC  27599-7295

Fax:  919-966-3015
Vos Lab web page:  http://www.med.unc.edu/wrkunits/3ctrpgm/lccc/VOSlab

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