Dear Human Genome Researchers,
Our laboratory has developed strategies to bridge contig gaps occurring
at human genomic regions which cannot be cloned and/or maintained
faithfully in bacteria using the large cloning systems (PI/BAC/PAC,
etc.). Such strategies derive from the original HAEC technology which
allows direct cloning of DNA in human cells as Human Artificial Episomal
Chromosomes (Nature Genetics 8:33-41, 1994; Methods in Molecular Genetics
8:167-188, 1996).
We have now entered the phase of testing such technology for
proof-of-concept. We will provide the human genome community with a
resource to isolate and sequence refractory contig gaps. Hence, we are
interested in hearing from the human genome community about persistent
and refractory genomic gaps in order to identify potential candidates for
the HAEC bridging technology. Suitable candidates are gaps which have
been thoroughly tested on highly redundant large bacterial and yeast
libraries and also shown to be missing in libraries prepared with
different restriction enzymes.
Interested human genome laboratories should send their e-mail reply to
the attention of Jean-Michel Vos at the following address:
angemari at gibbs.oit.unc.edu.
Dr. Jean-Michel H. Vos
349 Lineberger Comprehensive Cancer Center
School of Medicine CB#7295
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-7295
Fax: 919-966-3015
Vos Lab web page: http://www.med.unc.edu/wrkunits/3ctrpgm/lccc/VOSlab
