I am working on a 100kb BAC fragment and have subcloned several
repetitive and single copy sequences (all less than 3 kb). I would like to
make a restriction map of the entire BAC. Other than subcloning smaller
fragments (e.g. 10-50 kb) which could take a long time to dissect and order
does anyone have any suggestions for making a broad baseline map of the
entire BAC.
Thanks
Joe Miller
Department of Horticulture
University of Wisconsin-Madison
