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What is the smallest amount of carbon required for biofilm growth?

Jem Stach js9 at ukc.ac.uk
Wed Apr 8 16:42:13 EST 1998

Dear Biofilmers

I'm wondering if anyone could provide information on the minimum amount
of carbon required to allow biofilm growth. I'm aware that Lawrence et
al used 14 microgrammes of Diclofop, (equivalent to 7.8 microgrammes
total carbon)when growing biofilms in flow cells running at approx 2mL
per hour.

I am using the same flow cell system to view biofilms, but the compound
of interest solubility (in water)is about 0.8 microgrammes per mL.

I'm aware that I could adjust the flow rate to increase the amount of
carbon available over a given peroid of time; (information on the the
upper limits of shear stresses on biofilms would also be appreciated),
I'm interested to hear any comments on whether this amount of carbon is
able to support growth; and how to adjust solubility whilst staying
relevent to environmental models.  Any comments would be extremely


Jem Stach.

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