Fellow microbe-lovers,
I've been trying to do some fluorescent microscopy, and I have a
question about fixatives. When using 1% glutaraldehyde, I seem to be
getting autofluorecing cells when using a blue filter
Excitation wavelength: 450-490 nm
Dichroic mirror: 510 nm
Barrier filter: 515 nm (interference filter)
Has anyone else had this problem? Any references? Any suggestions for
other (less fluorescing) fixatives?
Any help or suggestions much appreciated.
-Mark
fuller at envirogen.com
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