We have used 35S to label bacteria for transport experiments in
laboratory sediment columns. However, our mass balances do not come out
to 100%, even after taken the decay rate of 35S into effect. What could
be the potential routes of loss for sulfur? The only one that come to
my mind is H2S, but we're not running under anaerobic conditions. What
doe the dissolved O2 or redox need to be to get H2S production. Also,
what would you suggest doing to "trap" any H2S that may be off-gasing
from the column? Is there any other sulfur compounds that would be
easily lost? We count the sediment wet, so any dissolved S-compounds
should be included in the scintillation counter results.
[These columns are run for about 20 days total]
Any suggestions would be greatly appreciated. Thanks in advance for
your time.
-Mark
fuller at envirogen.com
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