Cindy MORRIS wrote:
>> Dear colleagues,
> I need a little help in describing an ultrasonicator that we are using to
> break up biofilms in order to isolate the individual organisms within. I
> have submitted a manuscript in which we describe the ultrasonicator as
> follows:
>> ....Microbial suspensions were ultrasonicated for different times and
> intensities with a Vibracell-72405 100 Watt ultrasonicator (Bioblock
> Scientific, F-67403 Illkirch, France; diameter of ultrasonication tip, 6 mm
> ; maximum volume that can be sonicated, 25µl). All sonications were
> conducted with pulsations (1 sec off / 2 sec on). ...
>> The reviewer's comment was: "Sonicators are different the world over. If
> you want others to have a reasonable chance of floolwing your proceedure and
> confirming your results, you need to do a better job of describing the
> actual energy output of the probe under your working conditions."
>> In the papers we have looked at where ultrasonicators are used to break up
> biofilms, not much more (and sometimes even less) information is given about
> sonicator energy. Can anyone suggest some articles I can look at to get a
> better idea about how to describe our sonicator's energy output, or, in
> general, how this should be described.
>> Thanks
>> Cindy Morris
>>> Cindy E. Morris
>> INRA - Station de Pathologie Vegetale
> B.P. 94
> 84143 Montfavet, France
>> tel : (33) 490-31-63-84
> fax : (33) 490-31-63-35
> e-mail : morris at avignon.inra.fr>> -------------------------------------------------------------------
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Ms. Morris,
What the reviewer is looking for is the Watts of energy of the tip at
the setting you use, the frequency (probably in kiloHertz) of the tip
vibrations, and probably the total time of sonication. Indicating the
max volume is probably not important.
-Mark
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