Denture biofilms

[Bob McLean]

Jean,
There are a number of fluorescent probes which will measure redox 
potential, pH, Ca levels, viability, gram reaction, gene expression etc. 
Molecular Probes (a major manufacturer of such products - web site 
http://www.probes.com ) will give you technical data showing fluorescence 
correlation with e.g. pH or Ca levels. Even with this information, it is 
best if you perform correlation studies under your experimental conditions. 
Several people have published some very nice work in this area. Among 
others, I would look for papers by Zbignew Lewandowski and Gil Geesey 
(Montana State), Rob Palmer (University of Tennessee, Knoxville), Darren 
Korber and John Lawrence (Saskatoon, Saskatchewan, Canada), and the biofilm 
group in Lyunby Denmark (Claus Sternberg, Soren Molin, et al).
I hope this information is helpful.
Bob McLean
At 02:04 PM 7/11/99 -0500, you wrote: 
>Hella, 
>Working on denture biofilms, we want to study the physico-chemical 
>conditions that prevail within these biofilms. We sample intact denture 
>plaque by using cryosectioning embedding medium after freezing the denture. 
>By this way, we can obtain sections for microscopic observations. 
>Are there some probes or techniques that can be used to determine the 
>aerobic or anaerobic as well as pH status of fresh biofilms? 
>Thanks 
>Jean Barbeau 
>adjoint aux vices-doyens et responsable des études supérieures 
>Faculté de médecine dentaire 
>Université de Montréal 
>Montréal, Québec 
>CP 6128, Succursale Centre-Ville 
>H3C 3J7 
> 
> 
> 
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___________________________________________________________________________ 
R.J.C. (Bob) McLean, Ph.D. 
Dept. Biology 
Southwest Texas State University 
601 University Drive 
San Marcos, Tx 78666 
USA 
(512)245-3365 phone 
(512)245-8713 FAX 
Email: RM12 at swt.edu 
http://www.bio.swt.edu/micro/mclean/mclean.html

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