There is a very good little book that I don't think Tony mentioned. I
think it will answer most of your questions.
Title: Microbial Extracellular Polymeric Substances:
Characterization, Structure and Function
Eds: Jost Wingender, Thomas Neu, and Hans-Curt Flemming
Copyright: 1999
ISBN: 3-540-65720-7
Publisher: Springer-Verlag
Tony_Rook at steris.com ("Rook, Tony") wrote in message
news:<9hqi5f$ra$1 at mercury.hgmp.mrc.ac.uk>...
> Mr. Tiyawisutsri:
>
> There is a plethora of research in the area of the biofilm matrix otherwise
> referred to a the bacterial glycocalyx. Here are some pertinent references
> in this area (although there is much more):
>
>
> 1. Costerton JW, Irvin RT, Cheng KJ. The Bacterial Glycocalyx in
> Nature and Disease.
> Ann. Rev. Microbiol. 1981. 35: 299-324.
>
> 2. Marshall KC. Mechanisms of Bacterial Adhesion at Solid-Water
> Interfaces. In: Bacterial Adhesion: Mechanisms and Physiological
> Significance (eds. Savage DC, Fletcher M). Plenum Press, New
> York. 1985. pp 133-161.
>
> 3. Sutherland IW. Microbial Exopolysaccharides - Their Role in
> Microbial Adhesion in Aqueous Systems. CRC Critical Reviews in
> Microbiology. Volume 10, Issue 2: 173-201.
>
> 4. Allison DG. Biofilm-Associated Exopolysacharides. Microbiology
> Europe. Nov/Dec 1993. pp 16-19.
>
> As for your specific questions:
> 1. How to distinguish bacterial exopolysaccharide from biofilm
> exopolysaccharide?
>
> In the first above reference, Costerton et al defines the
> bacterial glycocalyx as "those
> polysaccharide- containing structures, of bacterial
> origin, lying outside the integral elements of the outer membrane of
> Gram- negative cells an the peptidoglycan of Gram-Positive cells"1.
>
> 2. Is EPS a minor component of biofilms?
>
> There are several studies which have investigated the
> exopolysaccharide content of biofilms. These should
> give you some idea of the content ratios of EPS to other
> biofilm components such as bacterial cells.
>
> 3. Are there specific biofilm exopolysaccharides?
>
> Although there are major polysaccharide groups involved in
> biofilm formation, much of the specific EPS composition is
> dependent on the microorganisms within the biofilm that is generating the
> EPS. The composition and ratios of different exopolysaccharide
> produced are not only dependent of the specific bacterial
> cell producing them but is also influenced by
> physical conditions ( i.e. influence of substratum, flowing
> conditions, and available nutrients). Much of this is
> detailed in the cited references listed above.
>
> Hope this helps,
>
> Tony A. Rook
> Scientist
> STERIS Corporation
> 5960 Heisley Road
> Mentor, OH 44060 USA
> Phone: (440) 392-7743
> Email: tony_rook at steris.com
>
>
> -----Original Message-----
> From: "rachaneeporn tiyawisutsri" [mailto:rachaneeporn_t at hotmail.com]
> Sent: Monday, July 02, 2001 5:15 AM
> To: biofilms at net.bio.net
> Subject: (NONE)
>
>
>
> Hello all, I am a graduate student in Medical technology at Mahidol
> University. I will have a seminar in " The biofilm matrix" . Please suggest
> me about the content. And I have some question about " How to distinguish
> bacterial exopolysaccharide from biofilm exopolysaccharide ?" ,
> Exopolysaccharide is the minor component of the bacterial biofilm? and the
> last question " Are there any specific biofilm exopolysaccharide ?
> Thanks for help
>
> Rachaneeporn Tiyawisutsri
> _________________________________________________________________________
> Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.
> ---
>
>
>
>
>
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