In response to the message from:
Rachaneeporn Tiyawisutsri
Graduate student in Medical technology
Mahidol University,Thailand
-----Original Message-----
From: Rook, Tony
Sent: Monday, July 09, 2001 8:57 AM
To: '"rachaneeporn tiyawisutsri"'
Subject: RE: (NONE)
Rachaneeporn:
I believe the "gold standard" for EPS detection and quantitiation are the
phenol-sulfuric and the anthron assays. Both use spectrophotometric
detection based on a colorimetric change. Both methods were initially
developed by chemists which wanted to quanitate different sugars. However,
the standard you use to generate a curve may vary depending on the the
specific polysaccharide you want to detect and/or the particular
organism(s) in the biofilm.
Here are some great references:
Anthron Assay:
Dreywood, R. Qualitative test for carbohydrate material. Ind Eng
Chem. 1946. 18, 499
Phenol-Sulfuric Assay:
Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F.
Smith. Colorimetric method for determination of sugars and related
substances. Anal. Chem. 1956. 28: 350-55.
Liu, D., P.T.S. Wong, and B.J. Dutka. Determination of
Carbohydrate in Lake Sediment by a Modified Phenol-Sulfuric Acid
Method. Water Research. Pergamon Press 1973. Vol. 7, pp
741-746. Printed in Great Britain.
General EPS References:
Spaeth, R., and S. Wuertz. Extraction and Quantitation of
Extracellular Polymeric Substances from Wastewater. In: Flemming,
H.C., U. Szewzyk, and T. Griebe (eds). Biofilms: Investigative Methods &
Applications. Technomic Publishing Company. Lancaster, PA
USA. 2000. pp 51-68.
Doutre, D.A., G.W. Hay, A. Hood, and G. W.
VanLoon. Spectrophotometric Methods to Determine Carbohydrates in
Soil. Soil Biol Biochem. Vol 10: pp 457-462. Pergamon Press Ltd
1978. Printed in Great Britain.
Blumenkrantz, N., and G. Asboe-Hansen. New Method for
Quantitative Determination of Uronic
Acids. Analytical Biochemsitry. 1973. 54: 484-489.
Hope this helps,
Tony A. Rook
Scientist
STERIS Corporation
5960 Heisley Road
Mentor, OH 44060
tony_rook at steris.com
-----Original Message-----
From: "rachaneeporn tiyawisutsri" [mailto:rachaneeporn_t at hotmail.com]
Sent: Sunday, July 08, 2001 12:11 PM
To: biofilms at net.bio.net
Subject: (NONE)
Hello ,
Thank you for your kindness. I have some question about " What is the gold
standard or reference method to detect or characterize biofilm
exopolysaccharide ? , and we can use lectin to detect or characterize
biofilm exopolysaccharide ?
Thanks for help
Rachaneeporn Tiyawisutsri
Graduate student in Medical technology
Mahidol University,Thailand
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