There will be a seminar on live cell imaging on Tues 12th Nov, followed by
wine and other refreshments.
Venue: Room LG92, Electron Microscope Unit (Madsen Building), University of
Sydney
<http://www.emu.usyd.edu.au/images/unimap.html>
Time: 16:00-17:00
LIVE CELL IMAGING: FROM LIGHT TO PROBE MICROSCOPY
Dr Filip Braet
Laboratory for Cell Biology and Histology, Free University of Brussels
(VUB), Laarbeeklaan 103, 1090 Brussels-Jette, Belgium
Scientists have long been interested in live cell imaging using traditional
light microscopic imaging methods. At that time, they were unable to
identify organelles, molecules or biochemical processes at the
(sub)cellular level. Within the last decade, however, combinations of novel
microscopic techniques with advanced electronic imaging and digital image
processing technology have resulted in dramatic improvement of the quality
of microscopic images, providing high-quality images or movies with a
resolution close to, or even below the theoretical limit of resolution.
Another major improvement is the recent availability of numerous
fluorescent or non-fluorescent probes specially designed for close in vitro
and in vivo monitoring of living cells. Today, molecules, proteins or
organelles hitherto invisible, can be clearly observed and measured, and
the recording of dynamic events, including molecular processes within
living cells has become routinely feasible. In the present talk we will
present a survey of the obligate conditions to be implemented during life
cell imaging over considerable periods of time: i.e., maintenance of
steady-state culture conditions regarding temperature, pH, CO2 and
osmolarity. The choice of optics, recording media and the use of contrast
methods together with the application of bioprobes will be discussed.
Finally, viability assessment of the cells during imaging remains an issue.
During the course of the presentation, the following microscopic methods
for cellular imaging will be outlined: time-lapse video microscopy, in vivo
microscopy (IVM), time-lapse (video) fluorescence microscopy, confocal
laser scanning microscopy (CLSM), and atomic force microscopy (AFM).
Accordingly, we will address some pronounced problems and artefacts
encountered during life cell imaging for each microscopic method presented.
In conclusion, the presented microscopical methods and conditions for life
cell imaging is not intended to be complete, but it comprises a selection
of examples to illustrate and summarize the basic strategies for their
application and their usefulness in the field of life sciences. Nowadays,
the modern microscope for life cell imaging is an indispensable tool in any
biological or biomedical laboratory interested in the structure and
dynamics of the living cell.
--
Dr Anya Salih
APD(I) Research Fellow
Electron Microscope Unit
& The Australian Key Centre for Microscopy & Microanalysis
Madsen Building FO9 Email: anya at emu.usyd.edu.au
The University of Sydney Telephone: 02-93517540
Sydney, 2006, AUSTRALIA Facsimile: 02-93517682
http://www.emu.usyd.edu.au/research/anya_research.html
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