Hi all,
I'm a postgraduate student working on imaging of Pseudomonas
aeruginosa biofilms.
After much reading, I came across 2 image analysis programs: COMSTAT
and ISA. From my understanding of these 2 programs, neither allows
quantification of biofilms using light microscopy. I do not have
fluorescent-labeled Pseudomonas aeruginosa thus is not able to utilize
COMSTAT. Unfortunately, apart from quantification of structural
parameters, ISA does not allow quantification of the whole biofilm.
Does anyone know of a program that can allow 3D analysis and
quantification of biofilm images taken using light microscopy?
I intended to label Pseudomonas aeruginosa with GFP. However, I lack
the means of transforming the plasmid into the bacteria since I do not
have appropriate conjugative E.coli strain S17-1. Is there any other
way to make the bacteria fluoresce throughout the whole experiment?
Please help! I'm at a loss of what can I do next.
---
-------------------------------------------------------------------
To reply to the group as well as to the originator, make sure that
the address biofilms at net.bio.net is included in the "To:" field.
See the BIOFILMS homepage at http://www.im.dtu.dk/biofilms for info
on how to (un)subscribe and post to the Biofilms newsgroup.
