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Trouble with imaging of Pseudomonas aeruginosa

Ashley Won g0305851 at nus.edu.sg
Thu Jul 29 03:45:04 EST 2004

Hi all,

I'm a postgraduate student working on imaging of Pseudomonas
aeruginosa biofilms.

After much reading, I came across 2 image analysis programs: COMSTAT
and ISA. From my understanding of these 2 programs, neither allows
quantification of biofilms using light microscopy. I do not have
fluorescent-labeled Pseudomonas aeruginosa thus is not able to utilize
COMSTAT. Unfortunately, apart from quantification of structural
parameters, ISA does not allow quantification of the whole biofilm.
Does anyone know of a program that can allow 3D analysis and
quantification of biofilm images taken using light microscopy?

I intended to label Pseudomonas aeruginosa with GFP. However, I lack
the means of transforming the plasmid into the bacteria since I do not
have appropriate conjugative E.coli strain S17-1. Is there any other
way to make the bacteria fluoresce throughout the whole experiment?

Please help! I'm at a loss of what can I do next.

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