Hi,
If you have problems culturing the organisms you could try a range of
different media from R2A agar and peptone water agar through to
nutrient and LB. I guess R2A might be best. Also, you could modify the
media, so that it better represents the environment from which the
bacteria (and possibly fungi? - these can be big players in the
colonization of plastics) originate.
A number of approaches could be employed to test for the efficacy of
antimicrobials - have you considered using poloxamer hydrogels? This
was done by Gilbert et al., (The use of poloxamer hydrogels for the
assessment of biofilm susceptibility towards biocide treatments. J
Appl Microbiol. 1998 Dec;85(6):985-90. ). Also, you could use agarose
beads (See papers by Phil Stewart from Montana Biofilm research
center, such as, Cochran WL, McFeters GA, Stewart PS. 2000. Reduced
susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen
peroxide and monochloramine. J Appl Microbiol. 2000 Jan;88(1):22-30).
Also consider using lectins to construct artificial biofilms (Rickard,
A.H. and Gilbert, P. 2003. Disinfection kinetics in artificial
bacterial aggregates. In Biofilm communities: Order from chaos? pp.
125-139. Edited by McBain, A.J., Allison, D., Brading, M., Rickard,
A.H., Verran, J. and Walker, J. Bioline Press, Cardiff, UK).
Alex
Alex Rickard
NIDCR
USA
"R=E1faga" <unimpeachable184 at hotmail.com> wrote in message
news:<10o84ij5mmiif6f at corp.supernews.com>...
> Hello. I am conducting research work for a small beer packaging firm here in
> the U.S. I would like to evaluate the effectiveness of various conveyor
> alkaline detergents and biocidal conveyor lubes, but I am having difficulty
> culturing these biofilms on solid or liquid media, since the difference in
> growth conditions renders a much different microbiological matrix by
> possibly excluding the emergence of some strains. I realize that I could
> perform comparative studies in-situ, but I would rather do it under more
> controlled conditions. Any ideas?
>
>
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