Dear Rachna,
While in theory any stain that would stain biological components of
biofilms (and not the underlying polystyrene substratum) could be
used, crystal violet absorbs much more light than safranin and so
would provide higher sensitivity for this assay.
Good luck with your work,
Bob McLean
At 09:15 AM 6/19/2007, Rachna Singh wrote:
>Why is crystal violet preferred over safranin for staining bacterial
>biofilms formed on the surface of microtiter plates?
>>_______________________________________________
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R.J.C. (Bob) McLean
Department of Biology
Texas State University
601 University Drive
San Marcos, TX 78666
USA
New University Name: Texas State University
(512) 245-3365 phone
(512) 245-8713 FAX
Email: McLean from txstate.edu
Website: http://www.bio.txstate.edu/~micro/mclean/mclean.html
