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[Biofilms] Looking for a reference for one of your suggested protocols

Stefan Moreira via biofilms%40net.bio.net (by samoreir from lakeheadu.ca)
Thu Oct 23 11:19:47 EST 2008

Hello Mr. Wright,

I'll begin by apologizing if I have addressed you incorrectly,  I came
across your name in a rather dated (1997) correspondence with George O'toole
when searching for a good way of enumerating the viable cells in a biofilm.
The method you describe to Mr. O'toole (employing sonication of cells
followeed by dilution) sounds perfect my intended application;enumerating
the E. coli cells from a biofilm in a microplate, and was hoping that you
may have on hand a reference for the protocol you described. Here is a link
to the description you provided
http://www.bio.net/bionet/mm/biofilms/1997-April/000106.html. I realize this
is a very long shot but figured I would try none-the-less. Thank you very
kindly in advance.


Stefan Moreira H.B.Sc.Bio.
M.Sc. Candidate
Lakehead University
Thunder Bay, ON
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