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immobilizing worms

Terese Rakow rakow at SCRIPPS.EDU
Fri Nov 4 19:47:07 EST 1994


        I will be starting injections in worms shortly and am interested in
ideas for "sticking" the worms down.  I have a lot of experience with
injections of eggs, but with those, the eggs were immobilized with
poly-L-lysine onto a glass coverslip.  I have gone through the procedures
of dessication, anesthetization, and immobilization under oil.  Are these
the only methods out there, or are there other sticky/viscous substances
that could be used?  Any comments or experiences would be appreciated
please post or e-mail directly to rakow at scripps.edu 
        Thanks in advance.
        Terese Rakow




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