Getting GFP tightly localized to the nucleus using the SV40 nuclear
localization signal poses a bit of a problem due to the small size of GFP.
Andy Fire found that a larger GFP fusion carrying beta-galactosidase at the C
terminus of GFP results in a GFP signal that is restricted to the nucleus (WBG
13:4, 30). However, the signal is weaker than with GFP alone; placing
beta-gal at the C terminus of GFP may diminish GFP activity. Has anyone found
another trick to keep GFP in the nucleus without losing the strong signal
(e.g., using multiple copies of GFP, placing it on the C terminus of beta-gal,
etc.)?
Joel Rothman rothman at biochem.wisc.edu
Dept. of Biochemistry +1 608 262 4994
University of Wisconsin fax 262 3453
