In article <n1426530487.44961 at QuickMail.Yale.edu>, SternM_Lab at QUICKMAIL.CIS.YALE.EDU ("SternM Lab") writes:
> Subject: Time:11:38 AM
> OFFICE MEMO Low stringency Hyb conditions Date:11/23/94
>> Hi fellow netters,
>> I want to probe genomic libraries from other Nematode species with my
> genomic fragment from C.elegans.
>> I have a lot of conflicting information as to how to go about doing this.
> I would specifically like to see protocols that have been successful for you
> or your lab. I would also like to know what you probed with (genomic, cDNA,
> oligo, etc.) and how you might have altered the protocol based on expected
> conservation.
>> I will happily post a summary of responses if people are interested.
>> Thanks for your time,
>> Rebecca Burdine
> I would be very interested in these answers also. I am getting ready to
probe a C. vulgaris and C. Briggsae genomic libraries with a C. elegans
cDNA probe. Not too sure what stringency to hybe at and Stratagene has
not responded to my request of a protocol.
john
