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Low stringency Hyb conditio

mcmanusj at omrf mcmanusj at omrf
Wed Nov 23 08:12:33 EST 1994

In article <n1426530487.44961 at QuickMail.Yale.edu>, SternM_Lab at QUICKMAIL.CIS.YALE.EDU ("SternM Lab") writes:
>                        Subject:                               Time:11:38 AM
>   OFFICE MEMO          Low stringency Hyb conditions          Date:11/23/94
> Hi fellow netters,
>      I want to probe genomic libraries from other Nematode species with my
> genomic fragment from C.elegans.  
>      I have a lot of conflicting information as to how to go about doing this.
> I would specifically like to see protocols that have been successful for you
> or your lab.  I would also like to know what you probed with (genomic, cDNA,
> oligo, etc.) and how you might have altered the protocol based on expected
> conservation.
>    I will happily post a summary of responses if people are interested.
> Thanks for your time,
> Rebecca Burdine
  I would be very interested in these answers also.  I am getting ready to
  probe a C. vulgaris and C. Briggsae genomic libraries with a C. elegans
  cDNA probe.  Not too sure what stringency to hybe at and Stratagene has
  not responded to my request of a protocol.  


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